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restriction endonuclease hin diii  (TaKaRa)


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    Structured Review

    TaKaRa restriction endonuclease hin diii
    Restriction Endonuclease Hin Diii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hin+diii/Hind+III/pmc13168525-109-18-22
    Average 99 stars, based on 9168 article reviews
    restriction endonuclease hin diii - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Construct:

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Cloning:

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Plasmid Preparation:

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Article Title: Validation of Recombinant Type I Interferon Antiviral Activity Against Porcine Epidemic Diarrhea Virus In Vitro and In Vivo
    Article Snippet: .. The collected PCR product and the pET32b (+) vector (Vazyme, Nanjing, China) were separately digested with Bam HI and Hin dIII (TaKaRa, Qingdao, China) restriction enzymes. .. The digested fragments were ligated, and then the chosen monoclonal constructs were subjected to sequencing to verify the correct construction of the recombinant plasmid, followed by transforming into RossettaTM (DE3) competent cells (Solarbio, Beijing, China).

    Article Title: Competitive binding of TeXlnR and TeAmyR regulates cellulase production in Talaromyces endophyticus
    Article Snippet: .. After digestion by Hin dIII (TaKaRa, Japan) and Kpn I (TaKaRa, Japan), the pSilent-1 vector forward and reverse fragments from TeXlnR / TeAmyR were inserted into the Hin dIII and Kpn I cleavage sites successively to yield the plasmid pSilent-1- TeXlnR /pSilent-1- TeAmyR . ..

    Article Title: Validation of Recombinant Type I Interferon Antiviral Activity Against Porcine Epidemic Diarrhea Virus In Vitro and In Vivo
    Article Snippet: .. The collected PCR product and the pET32b (+) vector (Vazyme, Nanjing, China) were separately digested with Bam HI and Hin dIII (TaKaRa, Qingdao, China) restriction enzymes. .. The digested fragments were ligated, and then the chosen monoclonal constructs were subjected to sequencing to verify the correct construction of the recombinant plasmid, followed by transforming into RossettaTM (DE3) competent cells (Solarbio, Beijing, China).

    Ligation:

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Polymerase Chain Reaction:

    Article Title: Validation of Recombinant Type I Interferon Antiviral Activity Against Porcine Epidemic Diarrhea Virus In Vitro and In Vivo
    Article Snippet: .. The collected PCR product and the pET32b (+) vector (Vazyme, Nanjing, China) were separately digested with Bam HI and Hin dIII (TaKaRa, Qingdao, China) restriction enzymes. .. The digested fragments were ligated, and then the chosen monoclonal constructs were subjected to sequencing to verify the correct construction of the recombinant plasmid, followed by transforming into RossettaTM (DE3) competent cells (Solarbio, Beijing, China).

    Article Title: Validation of Recombinant Type I Interferon Antiviral Activity Against Porcine Epidemic Diarrhea Virus In Vitro and In Vivo
    Article Snippet: .. The collected PCR product and the pET32b (+) vector (Vazyme, Nanjing, China) were separately digested with Bam HI and Hin dIII (TaKaRa, Qingdao, China) restriction enzymes. .. The digested fragments were ligated, and then the chosen monoclonal constructs were subjected to sequencing to verify the correct construction of the recombinant plasmid, followed by transforming into RossettaTM (DE3) competent cells (Solarbio, Beijing, China).

    Purification:

    Article Title: A Genome-Wide Mutant Screen Identifies XopN and XopX as Core Type III Effectors Required for Peach Infection by Xanthomonas arboricola pv. pruni
    Article Snippet: .. Genomic DNA of the mutants that showed reduced virulence on peaches were purified by using a Nucleospin Microbial DNA Kit (Takara Bio) and digested with Hin dIII, Xho I, Sph I, Xba I, or Kpn I (Takara Bio). ..



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